Polylysine Induces an Antiparallel Actin Dimer That Nucleates Filament Assembly
نویسندگان
چکیده
From the Research Service, Malcom Randall Department of Veterans Affairs Medical Center and the University of Florida College of Medicine, Gainesville, Florida 32608, the ¶Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, Florida 32610, the Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, and the **Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306
منابع مشابه
Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution.
An antiparallel actin dimer has been proposed to be an intermediate species during actin filament nucleation. We now show that latrunculin A, a marine natural product that inhibits actin polymerization, arrests polylysine-induced nucleation at the level of an antiparallel dimer, resulting in its accumulation. These dimers, when composed of pyrene-labeled actin subunits, give rise to a fluoresce...
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